Aereshaa: The thing is, I already know it would work for sure on a flash drive (as you can readily confirm) and I believe it would work on a floppy, but I was wondering if it were possible via a CD. A flash drive isn't exactly the most expendable of mediums, and a floppy drive isn't necessarily standard anymore.
However, in the end, a flash drive *would* work as a secondary choice, but then it's as if I were running it off the local drive anyways; I was hoping to find some cheeky way to disable the CD-ROM entirely, make the CD invisible, or corrupt the CD. On the flash drive, there might be a few problems with privileges/anti-virus software, but other than that I don't see any real obstacles/challenges. I'm beginning to think that approaching the registry or the drivers for the CD-ROM would be a better/more practical idea. I think that way would be less simple than making the CD unreadable/inaccessible, but likely a little bit more intuitive.
In the end, I'm not looking for anything irreversibly malicious (I'm testing it on my computer first), just something that would have the end result of a user only being able to run the video-file once. I know the hapless victim well, so I will just reverse it once I manage to pull off the prank...
Marwex: And of course, if I get it going, I will put together a tutorial outlining what I did and how it worked. I'm sure it is simple, but that doesn't mean it's easy. However, that might be because it's not actually possible and I just don't know it yet.
Xav: Methodz change of heart surprised me too... it must be the drugs

That being said, I'd be lying if I said I wasn't curious as to what he's going to whip up...